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Cell group

Prof. Takeo Yamaguchi

Research Topics

Biophysical chemistry of biomembrane

Current Research

  1. Molecular mechanism of pressure-induced hemolysis in erythrocytes
  2. Molecular mechanism of pressure-induced apoptosis in murine erythroleukemia cells

Biography

  • PostDoc, University of Pennsylvania 1988
  • Ph.D., Fukuoka University, 1984
  • M.A., Kyushu University, 1978
  • B.A., Saga University, 1971

Keywords

Erythrocyte membrane, hemolysis, apoptosis, high pressure

Assist. Prof. Hidehiko Tanaka

Research Topics

Antioxidant properties of amphiphilic ascorbic acid derivatives

Current Research

  1. Inhibition of paraquat cytotoxicity of amphiphilic ascorbic acid derivatives
  2. Antitumor activity of the combination of ascorbate and Cu/GlyGlyHis

Biography

  • PostDoc, University of Pennsylvania 1988
  • Ph.D., Fukuoka University, 1991
  • M.A., Fukuoka University, 1979
  • B.A., Fukuoka University, 1977

Keywords

Ascorbic acid, paraquat, antioxidant, tumor, Cu complex

Assist. Prof. Shigemi Nagatome

Research Topics

Colloid Chemistry of biosurfactants

Current Research

Multicomponent monolayers imitating human erythrocyte membrane

Keywords

Mixed monolayer, Cholesterol, plant sterols, Erythrocyte model membranes

Selected publication

  1. T. Yamaguchi, H. Kojima, S. Kawaguchi, M. Shimada, H. Aso, Papain cleavage of the 38,000-dalton fragment inhibits the binding of 4, 4’-diisothiocyanostilbene-2, 2’-disulfonate to Lys-539 on the 60,000-dalton fragment in human band 3, J. Biochem. 2017, doi: 10.1093/jb/mvx005
  2. K. Kiyotake, H. Ochiai, T. Yamaguchi, Agglutination of human erythrocytes by the interaction of Zn2+ion with histidine-651 on the extracellular domain of band 3, Colloids and Surface B: Biointerfaces. 2016, 141, 284-290
  3. K. Kiyoatake, S. Nagadome, T. Yamaguchi, Membrane perturbations induced by the interactions of zinc ions with band 3 in human erythrocytes, Biochem. Biophys. Reports, 2015, 2, 63-68
  4. T. Yamaguchi, K. Tajiri, K. Murata, S. Nagadome, Membrane damages under high pressure of human erythrocytes agglutinated by concanavalin A, Colloids and Surface B: Biointerfaces. 2014, 116, 695-699
  5. T. Yamaguchi, S. Miyauchi, Y. Isahara, Pressure-induced hemolysis of in vivo aged human erythrocytes is enhanced by inhibition of water transport via aquaporin-1, High pressure Research, 2013, 33, 285-291

Gene group

Assist. Prof. Masatora Fukuda

Research Topics

Molecular mechanism in regulating gene expression and gene function.
Design and synthesis of biomolecule controlling a target biological process.

Current Research

Molecular mechanism and physiological function of A-to-I RNA editing.
Molecular recognition mechanism between RNA editing enzyme and structure of substrate RNA.
Functional guide-RNA toward site-directed RNA mutagenesis.
Ribozyme for selective target RNA cleavage depending on RNA editing.

Biography

  • Asst. Prof., Kyoto University, 2010
  • PostDoc, Kyoto University, 2008
  • Ph.D., Kyoto University, 2007
  • M.A., Kyoto University, 2002
  • B.A., Kwansei Gakuin University, 2000

Keywords

Genetic information, RNA editing, RNA mutagenesis, Ribozyme

Selected publication

  1. Construction of a guide-RNA for site-directed RNA mutagenesis utilizing intracellular A-to-I RNA editing.
    Fukuda M, Umeno H, Nose K, Nishitarumizu A, Noguchi R, Nakagawa H.
    Sci Rep. 2017 7:41478. doi: 10.1038/srep41478.
  2. Identification of an RNA element for specific coordination of A-to-I RNA editing on HTR2C pre-mRNA.
    Fukuda M, Oyama Y, Nishitarumizu A, Omura M, Nose K, Deshimaru M.
    Genes Cells. 2015 20:834-46. doi: 10.1111/gtc.12272.
  3. Improved design of hammerhead ribozyme for selective digestion of target RNA through recognition of site-specific adenosine-to-inosine RNA editing.
    Fukuda M, Kurihara K, Yamaguchi S, Oyama Y, Deshimaru M.
    RNA. 2014 20(3):392-405. doi: 10.1261/rna.041202.113.
  4. Simultaneous detection of ATP and GTP by covalently linked fluorescent ribonucleopeptide sensors. Nakano S, Fukuda M, Tamura T, Sakaguchi R, Nakata E, Morii T. J Am Chem Soc. 2013 135(9):3465-73. doi: 10.1021/ja3097652.
  5. A strategy for developing a hammerhead ribozyme for selective RNA cleavage depending on substitutional RNA editing.
    Fukuda M, Kurihara K, Tanaka Y, Deshimaru M.
    RNA. 2012 18(9):1735-44. doi: 10.1261/rna.033399.112.

Protein group

Prof. Isao Kuraoka

Research Topics

Molecular mechanisms of mutagenesis

Current Research

My group is interested in studying mutagenesis in DNA and RNA. In particular, we are interested in DNA lesions, nucleotide excision repair, translesion DNA synthesis and RNA processing. We use biochemistry, molecular biology to find out the molecular mechanisms in these biological processes.

Biography

  • PostDoc, ICRF, 2001
  • Ph.D., Osaka University, 1996
  • M.A., Doshisha University, 1992
  • B.A., Doshisha University, 1990

Keywords

DNA lesion, DNA repair, Mutagenesis

Assist. Prof. Narumi Shioi (Aoki)

Research Topics

Elucidation of biodiversity and the molecular mechanism
-Adaptability of venomous snake by specific mutation (Site-directed mutagenesis)-

Current Research

  • Environmental adaption by a mutagenesis and the analysis of molecular machanuism.
  • Discovery novel functional proteins and peptides from venomous snake and applicatons thereof.

Biography

  • Ph.D., Fukuoka University, 2008
  • M.A., Fukuoka University, 2005
  • B.A., Fukuoka University, 2003

Keywords

Snake toxin, Environmental adaption, Biological defense mechanism

Selected publication

  1. Human endonuclease V is a ribonuclease specific for inosine-containing RNA.
    Morita Y, Shibutani T, Nakanishi N, Nishikura K, Iwai S, Kuraoka I.
    Nat Commun. 2013 4:2273. doi: 10.1038/ncomms3273.
  2. XPG stabilizes TFIIH, allowing transactivation of nuclear receptors:
    implications for Cockayne syndrome in XP-G/CS patients.
    Ito S, Kuraoka I, Chymkowitch P, Compe E, Takedachi A, Ishigami C, Coin F, Egly JM, Tanaka K.
    Mol Cell. 2007 26(2):231-43.
  3. Removal of oxygen free-radical-induced 5',8-purine cyclodeoxynucleosides from DNA by the nucleotide excision-repair pathway in human cells.
    Kuraoka I, Bender C, Romieu A, Cadet J, Wood RD, Lindahl T.
    Proc Natl Acad Sci U S A. 2000 97(8):3832-7.
  4. Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii).
    Shioi N, Deshimaru M, Terada S.
    Biosci Biotechnol Biochem. 2014 78(3):410-9. doi:10.1080/09168451.2014.890030.
  5. Small serum protein-1 changes the susceptibility of an apoptosis-inducing metalloproteinase HV1 to a metalloproteinase inhibitor in habu snake (Trimeresurus flavoviridis).
    Shioi N, Ogawa E, Mizukami Y, Abe S, Hayashi R, Terada S.
    J Biochem. 2013 153(1):121-9. doi: 10.1093/jb/mvs127.